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Selected reproducible workflows, scripts and teaching resources for phylogenomics, transcriptomics, UCE processing and sequence-data quality control.

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Python

Alignment_static_recovery

Recovery and validation utilities for static phylogenomic alignments.

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Pipeline

COI_Recovery_Pipeline_using_MitoGeneExtractor_and_ORF-based_Filtering

Reproducible workflow to recover mitochondrial COI sequences with ORF-based filtering.

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Shell

Treinamento_Processamento_UCE_UNESP_2025

Training material for bioinformatics and processing of ultraconserved elements (UCEs).

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Shell

Trim_UCE_reads_trimmmomatic

Tutorial and scripts for trimming UCE reads with Trimmomatic.

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Shell

Ortholog-Identification-and-Positive-Selection-Analysis-in-Single-Copy-Genes

Workflow for ortholog identification and positive-selection analyses in single-copy genes.

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Tutorial

Transdecoder_tutorial

Practical guide for coding-region prediction with TransDecoder.

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Shell

Comparative_Transcriptomics_Triatominae

Reusable workflow for comparative transcriptomics in Triatominae.

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R

tanglegram-methods

R methods and examples for comparing phylogenetic trees with tanglegrams.

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Pipeline

mitogenomas_PCGs_pipeline

Pipeline for mitogenome and protein-coding gene phylogenomics.

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Python

Charset-converter-for-IQ-TREE-

Utility for converting charset/partition definitions for IQ-TREE workflows.

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Python

Alinhament_view

Presence–absence matrices and heatmaps for phylogenomic alignments.

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Workflow

Genes_tree_with_Asteroid

Workflow for species-tree inference with Asteroid from gene trees.

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QC

Check_FastQC_MultiQC

Quality-control workflow using FastQC and MultiQC.

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Shell

assembly_quality

Scripts for summarizing and checking sequence-assembly quality.

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Python

make_astral_map

Python utility to generate ASTRAL-compatible map files from FASTA and NEXUS datasets.

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Python

generate_assembly_conf.md

Generates PHYLUCE assembly.conf files from cleaned-read directories.

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Workflow

ML-with-iqtree3

Guidelines and scripts for maximum-likelihood phylogenetic inference with IQ-TREE 3.

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Python

Cluster_Occupancy_Analyzer

Evaluates taxon occupancy in ortholog clusters from phylogenomic pipelines.

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Python

Alignments_metrics

Quantifies multispecies FASTA alignments and produces summary tables.

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Shell

assembly_UCE

Tutorial for assembling UCE datasets with PHYLUCE on SLURM systems.

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